3-IAA和FIRINOX治疗可降低自噬活性。a，用R菌群定植gnotobio小鼠，原位注射KPC细胞。未治疗或给予FIRINOX、NAC(第9-13天)或FIRINOX + NAC(各5只)治疗。显示实验第20天的肿瘤重量。b，使用+/−3-IAA替代原位KPC肿瘤的SPF小鼠，用FIRINOX治疗，并根据指示进行分析。包含IHC,免疫组织化学。c，原位肿瘤用苏木精和伊红(H&E)、LC3B或Ki67染色的代表性图像(左)及各野阳性细胞数(各n = 5个)(右)。比例尺，50 μm。d,e，将GFP-LC3B-RFP报告细胞株Hy19636_GLRM注射到SPF小鼠，并按指示处理小鼠(n = 5只)。该图显示了FIRINOX治疗后第一天(d)的GFP/RFP比值和第3天(e)的肿瘤重量。 Representative merged immunofluorescence images or indicated areas with a magnification of 3× are shown; scale bars, 10 μm. f, KPC cancer cells were orthotopically injected into R-microbiota-colonized mice and the indicated treatment was applied as shown in the scheme (n = 4 or 5). Tumor weight is shown at day 18 of the experiment. g, Tumor weight of orthotopic KPC tumors from gnotobiotic mice colonized with NR microbiota is shown nine days after the indicated treatment (n = 4 or 5). CQ, hydroxychloroquine. One experiment (c) or one out of two independent experiments (a,d,e–g) is shown. Each symbol represents one mouse. Error bars indicate s.e.m. Significant P values are indicated and were determined by one-way ANOVA followed by Dunnett’s (a,c,f) or Tukey’s (d,e) post-hoc test or Kruskal–Wallis test followed by Dunn’s post-hoc test (g). Credit:自然(2023)。DOI: 10.1038 / s41586 - 023 - 05728 - y
