Spns2删除影响淋巴细胞监禁和淋巴结的血管结构。代表共焦图像的T细胞区(CD3-green)和B细胞区(B220-magenta)在宫颈LNs WT, Spns2−−和Spns2-Mx1Cre老鼠。每个基因型n = 3。B T细胞和B细胞的监禁的LNs Spns2−−和Spns2-Mx1Cre小鼠紊乱比WT,发现更多的T细胞在基因敲除小鼠的B细胞区。每个符号代表一个图像从三个老鼠每个基因型。数字显示P值。使用单向方差分析。C颈LNs WT、组织化学染色Spns2−−, Spns2f / f(没有Mx1Cre)和Spns2-Mx1Cre老鼠台面式晶体管- 79。箭头显示的内部膜WT的戊肝病毒和Spns2f / f老鼠。每个基因型n = 3。 D Quantification of Meca-79 density in cervical LNs from WT, Spns2−/−, Spns2f/f (without Mx1Cre), and Spns2-Mx1Cre mice with MECA-79. ns not significant; t test. E Meca-79 localization was dysregulated in HEVs of Spns2−/− and Spns2-Mx1Cre mice. Meca-79 (red) and CD31 (green) antibody. F LYVE-1 staining of the lymphatic vessels in inguinal LN of indicated genotypes. G Quantification of density of lymphatic vessels in the subcapsular regions of LNs of Spns2−/− and Spns2-Mx1Cre mice and control mice. Each symbol represents one image from three mice for each genotype. ns not significant; t test. Credit:细胞和分子生命科学(2022)。DOI: 10.1007 / s00018 - 022 - 04573 - y
