POGZ损耗导致的损失ESC自我更新。()卡通描绘gRNA目标网站在老鼠Pogz基因外显子2;(底部)基因分型结果显示Pogz基因的突变等位基因1 (Mut1)。B存在显示Pogz表达突变的ESCs的减少。C在控制和免疫印迹分析POGZ POGZ−−ESCs。D双如果染色OCT4、POGZ表明OCT4水平通道突变的ESCs月初没有显著改变。E的细胞生长增殖缺陷的治愈Pogz−−和Pogz + /−ESCs相比的ESCs的控制。ESCs Mut1通道12使用分析。F代表图像显示形态控制,后期通过Pogz−−/ ESCs(通道号码是18)。白色箭头指向夷为平地分化细胞。 G Alkaline phosphatase staining of control and Pogz−/− ESCs. White arrows pointing to cells with reduced AP activities. H The mRNA expression levels of representative pluripotency-related, mesodermal, neuroectodermal, endodermal genes in control and Pogz−/− ESCs. I IF staining of GATA6 showing that GATA6 was abnormally expressed in Mut1 Pogz−/− ESCs. Restoring POGZ rescued the abnormal expression of Gata6. J WB showing that OCT4 levels were reduced in Pogz−/− ESCs, which can be reversed by reintroducing FLAG-POGZ. Bar: 25 μm. K Volcano plot showing the up- and down-regulated genes in the control and early passage Pogz−/− ESCs. The RNA-seq experiments were repeated two times. L GO analysis of up-regulated DEGs. M GO analysis of down-regulated DEGs. All WB and IF were repeated at least two times. qRT-PCR was repeated three times. Credit:分子自闭症(2022)。DOI: 10.1186 / s13229 - 022 - 00502 - 9
