无花果。1:分布的游离脂肪酸(FFA)和磷脂的老鼠的大脑。32的每个动物在这项研究中,使用组织被切割基底外侧杏仁核(BLA),中央杏仁核(CeA)前脑的前额叶皮层(PFC)、腹侧海马(VH),背侧海马(DH)和小脑(CB),甲苯基上的虚线表示的紫罗兰色与相应的前囱坐标的大脑部分。比例尺= 1毫米。b使用诊断离子识别和量化磷脂分散在液相色谱质谱(LCMS)。所示是一个假设的磷脂-模式分裂质谱。父母(M), sn-1 (R1)和sn-2 (R2)碎片离子质量是独特的物种,并用于多反应监测(MRM,插图)LCMS量化丰富。c使用游离脂肪酸FFA的示意图表示分析稳定同位素标记(FFAST)。对于一个给定的大脑区域从动物提取的远期运费协议从不同的实验条件(盐水配对、盐未配对、CPP配对,CPP未配对,请参见图2)单独标记的羧基端使用ffast - 124或ffast - 127。样本,掺入了ffast - 138标签内部标准,由LCMS和分析。每个物种FFA显示相似的3标签变异色谱洗脱时间,和每个FFAST片段的比例相对于丰富的内部标准片段允许量化FFA的条件。 This workflow was repeated 8 times, to establish FFA abundance in each of the 8 animals used in each experimental condition. d–i Profile measurements of FFAs and 5 classes of phospholipids (PA—phosphatidic acid, PC—phosphatidylcholine, PE—phosphatidylethanolamine, PG—phosphatidylglycerol, PS—phosphatidylserine) across the brains of the control (saline unpaired) rats from auditory fear conditioning experiments, with analytes shown by acyl chain composition. Bars represent the total analyte measurement, with coloured sub-bars corresponding to the mean individual analyte concentrations (pmol/mg tissue) observed across 8 animals. Error bars represent the cumulative standard error of the mean (SEM) for all analytes. Source data are provided as a Source Data file. From: Saturated free fatty acids and association with memory formation