小叶肾类器官包含独特的肾脏结构。(a)生成肾类器官的程序示意图。(b)双眼观察第8代iPSC球状体(a.，b.)和第21代肾脏类器官UMK2 (c.，d.)的概况。A.光学显微镜下4倍放大。比例尺描绘500 μ m。B.光学显微镜下10倍放大。比例尺描绘200µm。C. 1倍放大。标尺表示2000 μ m。D. 4倍放大。 Scale bar depicts 2000 µm. (c) Morphology of organoid section via histological H&E staining. A typical glomerulus-like structure is depicted by a dashed rectangle. Tubule-like structures are marked with an asterisk. Scale bar depicts 50 µm. (d) Confocal pictures of glomerular (ACTN4, yellow) and tubular (LTL, green) (OCT2, red) structures in UMK1 sections. A glomerulus-like structure was marked with an arrow and the membrane of tubular structures were marked with a dotted line. Scale bar depicts 50 µm. (e) Monitoring of iPSC spheroids and kidney organoids in a dextran uptake assay. a. iPSC spheroids treated with dextran after 4 h pulse. b. Kidney organoids treated with dextran after 4 h pulse. c. Kidney organoids treated with dextran after 24 h chase. Scale bar depicts 200 µm. Credit:细胞(2022)。DOI: 10.3390 / cells11040635
