图1:ASH1L表达和H3K4me3水平明显降低后期的PFC ASD患者,由可拆卸的复制的ASH1L PFC的年轻老鼠。定量PCR数据显示ASH1L mRNA水平后期PFC组织从控制人类与特发性ASD患者。n = 12人(10 M, 2 F) /组* * p < 0.01, t双尾检验。b免疫印迹数据显示H3K4me3 H3K36me2级别在后期PFC控制与ASD患者。n = 12人(10 M, 2 F) /组* p < 0.05, t双尾检验。c定量PCR数据显示Ash1L N2A mRNA水平细胞转染Ash1L成分或炒(sc)成分。n = 6 /组。* * * p < 0.001,双尾t测试。d共焦图像显示病毒感染PFC地区(与DAPI染色,蓝色)从一只老鼠的立体定位注射Ash1L shRNA AAV (GFP-tagged)。酒吧规模:300μm。 e, f Quantitative PCR and Western blot data showing Ash1L mRNA and protein levels in PFC of mice (5-week-old) with the stereotaxic injection of Ash1L shRNA vs. sc shRNA AAV. Ash1L mRNA, n = 15 mice(8 M,7 F) for sc shRNA group, n = 14 mice(7 M,7 F) for Ash1L shRNA group; Ash1L protein, n = 8 mice(4 M,4 F)/group, ***p < 0.001, two-tailed t test. g Western blot data showing H3K4me3, H3K36me2, and H3K36me3 levels in PFC infected with Ash1L shRNA or scrambled shRNA AAV. n = 8 mice (4 M,4 F)/group, ***p < 0.001, two-tailed t test. h Representative confocal images and quantification of immunostaining of H3K4me3 (red) in PFC neurons infected with Ash1L shRNA or a scrambled shRNA AAV (GFP+ , green). n = 20 images/4 mice (2 M,2 F)/group, ***p < 0.001, two-tailed t test. Scale bar: 10 μm. i, j Representative confocal images and quantification of immunostaining of NeuN (red) and DAPI (blue) in PFC neurons infected with Ash1L shRNA or a scrambled shRNA AAV (GFP+ , green). Slices were collected at 8–9 days postinfection. n = 20 images/4 mice (2 M,2 F)/group. Scale bar: 20 μm. All the full Western blots are included in Supplementary Fig. 7a–c. Data are presented as mean values ± SEM. Detailed statistical data are provided in a Source Data file. Credit: DOI: 10.1038/s41467-021-26972-8
