功能性神经解剖学LPB预测腹侧中脑。实验设计。b,整个大脑荧光图像显示LPBVGLUT2预测(eYFP,绿色;上一行,水平;较低的行,矢状)。VTA、信噪比和东航以不同的颜色突出显示(比例尺1毫米)。c, LPBVGLUT2终端和纤维的通道(白色箭头)在不同腹侧中脑亚区(比例尺,50μm)。d-f(左)CAV2-Cre注入信噪比(d), VTA (e)和CeA (f) Ai14小鼠(n = 3老鼠为每个投影目标)。中间,逆行标记神经元(tdT积极、红色)在有限目的银行模式不同亚区(DAPI,蓝色;酒吧,规模100μm)。 Right, quantification of retrogradely labeled cells for different LPB subregions. g, Experimental design. h, EPSCs generated at −70 mV by light stimulation of LPBVGLUT2 inputs to VTA DA neurons (that is, TH immunopositive; Extended Data Fig. 1t) projecting to NAcLat (red trace) or non-DA (that is, TH immunonegative; Extended Data Fig. 1u) cells in the SNR (black trace). i, Mean EPSC amplitudes produced by light stimulation of LPBVGLUT2 inputs to different cell populations (DA → NAcLat, n= 21 cells; SNR, n= 25 cells; recorded in artificial cerebrospinal fluid (ACSF)). j, Application of 20 µM CNQX and 50 µM APV blocked EPSCs in SNR cells (n= 16 cells) and NAcLat-projecting DA neurons (n= 7 cells). k, Experimental design (left) and EPSC amplitudes produced by light stimulation of excitatory LPB inputs onto GAD2-tdT-positive VTA neurons (right; VTAGAD2+, n= 10 cells; recorded in ACSF; Extended Data Fig. 1v). l,m, Spontaneous firing from NAcLat-projecting VTA DA neurons (l) and SNR cells (m) in response to 10-Hz light stimulation of LPBVGLUT2 inputs. LPBVGLUT2 stimulation significantly increased firing of both NAcLat-projecting DA neurons and SNR cells (NAcLat, n= 12 cells; SNR, n= 12 cells). Significance was calculated by means of a paired t-test within-group comparison (j), an unpaired t-test (i) or one-way repeated-measures (RM) ANOVA with Tukey’s post hoc test (l and m). **P Credit: Yang et al.
