S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Credit: Scientific Reports (2020). DOI: 10.1038/s41598-020-57971-2">
S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Credit: Scientific Reports (2020). DOI: 10.1038/s41598-020-57971-2" width="800" height="486">
Jedi在外周胶质胶质和内皮中表达。（一种）成人的横截面（8-12周龄）为Jedi-1（洋红色）和DAPI（蓝色）染色的WT坐骨神经。插入1和2显示血管中的Jedi表达。请参阅补充图。S2用于验证脑胶质胶质细胞中的JEDI-1表达。（B.）WT P0 DRG为JEDI-1（红色），卫星胶质标记BFABP（绿色）和DAPI（蓝色）共染成。（C）WT DRG为Jedi-1（红色）和PGP9.5（绿色）和TOPRO3（蓝色）共染成。右侧显示插图。（D.）右：使用TUJ1（绿色）和JEDI-1（红色）染色神经元的主要WT DRG培养物。左：过表达小鼠Jedi-1的HeLa细胞被用作Jedi-1免疫细胞化学的阳性对照体外。在imagej 2.0.0-RC-69 / 1.52P中分析所有图像。信用：科学报告（2020）。DOI：10.1038 / S41598-020-57971-2
S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Credit: Scientific Reports (2020). DOI: 10.1038/s41598-020-57971-2" width="800" height="486">
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