生病时全脑活动的变化。a，样本采集和后续FOS全植入脑成像的时间线。在生理盐水或LPS (0.5 mg kg)后的指定时间点收集样本(圈出的数字)−1)注入。除LPS后24 h n = 3外，其余各组n = 4。b，每个时间点LPS与生理盐水在指定区域的log2[倍变化]热图。只有在某个时间点至少有一个显著变化的区域(经假发现率调整后P < 0.05)才显示。左侧色条，大脑细分;CTXsp，皮层底板;STR,纹状体;朋友,螺旋体;MB,中脑;AP和NTS被指明。 c,d, Representative images of FOS expression 3 h after saline (c) and LPS (d) injection. The right panels show a higher-magnification view of the NTS–AP region outlined in the left panels. Scale bars, 1 mm (left) or 250 μm (right). e, From left to right: the NTS and AP in the Allen Brain Atlas (ABA), ClearMap output of significant changes (false discovery rate-adjusted P < 0.05, red) for post LPS (3 h) versus saline injection, ClearMap-generated average heatmap of FOS counts for saline injection (n = 4), and ClearMap-generated average heatmap of FOS counts for LPS injection (3 h, n = 4). f,g, FOS counts for the NTS (f) and AP (g), for the indicated treatments (LPS concentration, 0.5 mg kg−1)和时间点;ClearMap的输出。错误条代表s.e.m.。鼠标插图是使用BioRender.com创建的。信贷:自然(2022)。DOI: 10.1038 / s41586 - 022 - 05161 - 7
