图1:SARS-CoV-2感染是大脑中与字符串血管增加有关。得了,SARS-CoV-2-infected患者的大脑中,空基膜管,也称为字符串船只(箭头),额叶皮层的增加。第四部分为基底膜染色标记胶原蛋白(胶原IV)和内皮细胞CD34标记。在a和b代表图像得到的数据集c,酒吧,规模50μm。b,放大的z-stack字符串的最大投影船与正交视图来排除这些局部的毛细血管。规模的酒吧、3μm。c,每张图象量化弦血管的体积。N = 23控制病人,N = 17 COVID-19病人。d,疣状显示更多的活跃caspase-3-positive船只SARS-CoV-2-infected皮质部分的病人(N = 6)比对照组(N = 6)。代表图像和量化。 Scale bar, 20 µm. e,f, SARS-CoV-2-infected hamsters developed an increased number of string vessels as shown by co-staining for coll IV and the endothelial marker caveolin-1. e, Representative images of coll IV and caveolin-1 in the cortex of hamsters 4 d post infection (p.i.) with SARS-CoV-2 and of uninfected hamsters. Scale bar, 50 µm. f, Quantification of string vessel lengths as a percentage of total vessel length in SARS-CoV-2-infected hamsters at 4, 7 and 24 d p.i. and in uninfected controls (N = 4 hamsters per group). g,h, SARS-CoV-2-infected K18-hACE2 mice developed an increase in string vessels as shown by co-staining for coll IV and caveolin-1. g, Quantification of string vessel lengths as a percentage of total vessel length in SARS-CoV-2-infected K18-hACE2 mice 2 d p.i. (N = 3 mice) and 7 d p.i. (N = 3 mice) and in uninfected controls (N = 5 mice). h, Representative images of coll IV and caveolin-1 in the cortex of K18-hACE2 mice 7 d p.i. and of uninfected K18-hACE2 animals. Scale bar, 50 µm. *P < 0.05, **P < 0.01. Means ± s.e.m. are shown. N denotes the number of patients or animals. Credit: DOI: 10.1038/s41593-021-00926-1
