P2小鼠左心室尖切除后的瘢痕和新的心肌组织评估显示心肌再生缺乏。(A)截肢后P2小鼠存活(AMP;n = 91)或不(SHAM;N = 51)左心室心尖的一小部分。术后即刻(0 h)、术后3 h (3 h)、术后24 h (24 h)测定动物存活。数据均为平均值±SEM。(B) 21 dpa时AMP和SHAM心脏的立体显微镜照片显示术后21天室外壁上存在广泛的白色斑块(黑色箭头)。(C)术后不同时间点分别测定P2 AMP (n = 3)和SHAM (n = 3)小鼠的心脏重量。(D) 21 dpa P2心脏代表性的H&E和三色染色切片。心肌组织丢失的明显表现为组织纤维排列失调(H&E插图)和强烈的蓝色染色(三色插图)。虚线表示病灶边界。 Scale bar, 200 μm. Right: Quantification analysis of the scar area in P2 AMP versus SHAM at 21 dpa. ***P < 0.001. (E) Paraffin-embedded resected P2 hearts were sectioned and immunostained for troponin-I and DAPI. Representative images at various time points during the post-AMP process demonstrate an absence of newly formed myocardial tissue. An asterisk indicates the healing area, whereas the dashed line marks the lesion border. Scale bar, 25 μm. (F) Number of proliferating CMs (DAPI+/MF-20+/BrdU+ cells) per 0.1 mm2 counted in paraffin-embedded SHAM and AMP heart sections from BrdU multiple pulse-chase experiments at the indicated dpa. Data are means ± SEM; n = 3. Only fields completely filled with cells were included in the analysis. ns, not significant. Credit:科学的进步（2018）。DOI：10.1126 / sciadv.aao5553
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