免疫组织化学的人类无法和TempO-Seq OB和OT的转录组分析。(a - c)包含IHC 6控制和6该人的时尚主题。(A)的污点是用来清楚地说明在OT高低有髓鞘的地区。条形图表示的意思是表面高低有髓鞘的每个区域,表示为总面积的百分比分析。一个方差分析产量无显著差异的基因型F = 0.1, 1 d.f。,p > 0.05,显著区别高低髓鞘形成领域F = 26.84, 1 d.f。(p < 0.001)。(B)代表图像和量化的AβOT从6 Ctrl和6时尚的病人。Aβ正面表面增加时尚患者控制相比,有髓不高和低。在时尚集团Aβ较高的积累比低有髓不高有髓。方差分析产量的显著差异基因型F = 13.94, 1 d.f。, p<0.01 and a significant difference between high and low myelination areas F=17.22, 1 d.f., p<0.001. (C) Quantification of microglia/macrophage markers Iba1 and CD68, showing increased neuroinflammation in the high myelinated OT. ANOVA for Iba1 and CD68 yields a significant difference for genotype (Iba1, F=13.54, 1 d.f., p<0.01, CD68, F=23.24, 1 d.f., p<0.001). (D and E) Quantification of overlap in expression of Aβ, Iba1 and CD68. (D) Quantification of the 3 different microglial populations: Iba1+CD68+ (arrows “1”), Iba1+CD68- (arrows “2”), Iba1-CD68+ (arrows “3”). An ANOVA yields a significant difference for the genotype for Iba1+CD68+ (F=14.1, 1 d.f., p<0.01), Iba1+CD68- (F=8, 1 d.f., p<0.05) and Iba1-CD68+ (F=6.65, 1 d.f., p<0.05). FAD patients exhibit an increase in Iba1+CD68+ surface and Iba1+CD68- surface in the high myelinated OT, and an increase in Iba1-CD68+ surface low myelinated OT, compared to Ctrl. (E) Quantification of Aβ surface that is overlapping with Iba1+CD68+ cells. An ANOVA yields a significant difference for the genotype for Aβ surface that is overlapping with Iba1+CD68+ cells (F=24.14, 1 d.f., p<0.001), Iba1+CD68- cells (F=9.93, 1 d.f., p<0.01) and Iba1-CD68+ cells (F=12.28, 1 d.f., p<0.01). An ANOVA yields a significant difference between high and low myelination areas for Aβ surface that is overlapping with Iba1+CD68- cells (F=8, 1 d.f., p<0.05) and Aβ free from microglial coverage (F=15.93, 1 d.f., p<0.001). Aβ surface that is overlapping with all three microglial populations is increased in the high myelin OT of FAD patients compared to Ctrl. Aβ surface that is overlapping with Iba1-CD68+ cells is increased in the low myelin OT. Aβ free from microglial coverage (arrows “4”) is decreased in the low myelin OT compared to the high myelin OT in both Ctrl and FAD. Scale bar: 50 µm. Credit:神经生物学衰老的(2022)。DOI: 10.1016 / j.neurobiolaging.2022.12.004
