诱导和维持SOX1积极背前脑神经上皮的细胞。神经诱导方案hPSCs TGFbR和BMPR抑制剂的存在。后皮质神经细胞感应NES SOX2表达巢蛋白阳性细胞b细胞SOX1 TUBB3负面c, FOXG1 d, e PAX6 SOX2阳性细胞。实验重复5生物独立的细胞系。Scalebar: 25μm f SOX1阳性细胞比例在通道5文化他(H1、H9 CA1)派生cNESCs处理各种因素的组合(n = 3独立的细胞系,10点/每个细胞株/组,红酒吧意味着±SEM,单向方差分析,图基的测试)。cNESCs g规范化模型的细胞数量变化(数据提出了均值±SEM的H1 - H9、CA1-derived细胞,n = 3独立的细胞系,双向方差分析,Dunnett测试)超过10通道(30天)。h相衬的形象H1派生cNESCs (p36) 4天4 f(上)和14天的文化(底部)形成莲座状结构。实验重复了4生理上独立的细胞系。Scalebar: 50μm。我:集落形成试验的cNESCs培养在播种前4 f 200细胞/ cm2密度。 j Schematic presentation of developmental signaling pathway components targeted in our assay, protein ligands are in bold and chemical inhibitors are in italic, 4F components are in red. k Quantification of cell number changes after 96-h treatment of cNESCs with indicated ligands or chemical inhibitors compared to 4F condition (n = 3 independent experiments, data are presented as mean ± SEM, 1 way ANOVA, Tukey’s test, white bars are p < 0.01, grey bars are not significantly different from control, red line indicates starting cell number). Credit:自然通讯(2022)。DOI: 10.1038 / s41467 - 022 - 29839 - 8
