MCH增强dLS中的兴奋性突触传递。a, RNAScope对MCHR1和vGAT的原位杂交结果。b，通过对表达ChR2的MCH轴突进行20 hz、2 min光遗传刺激之前(对照)和之后的场刺激，dLS-LHA神经元中ampar介导的(Vh =−70 mV)和nmda介导的(Vh =+40 mV) EPSCs的样本痕迹。c，显示AMPAR-EPSCs (n= 11/8，神经元/小鼠)、NMDAR-EPSCs (n= 8/6，神经元/小鼠)振幅和AMPAR-EPSC/NMDAR-EPSC (n= 8/6，神经元/小鼠)比值的池化归一化数据。所分析的神经元/动物数量以柱状表示。采用配对双尾学生t检验:AMPAR-EPSC, P= 0.0057;Nmdar-epsc, p = 0.0064;AMPAR/NMDAR比值，P= 0.0508。d，在长时间光遗传刺激前后，用成对的场刺激脉冲叠加诱发的EPSCs样本痕迹。e, PPR和诱发EPSCs归一化振幅的汇总数据(n= 7/6，神经元/小鼠)。 Paired two-tailed Student’s t-tests were used, P= 0.0014. f, shRNA knockdown of Pmch in the hypothalamus in MCH-Cre mice. Viruses were injected into one side. g, AAV-DIO-C1V1 injection-labeled MCH neurons (arrow and arrowhead). shRNA-infected neurons show no expression of MCH (arrowhead). h, Representative evoked EPSCs and pooled data in control (shRNA-scramble). i, Representative evoked EPSCs in MCH knockdown (shRNA-Pmch) animals. j, Pooled data of evoked EPSCs and PPR in scramble shRNA group, n= 9/4, neurons/animals. Paired two-tailed Student’s t-tests were used, P= 0.004 for eEPSC and P= 0.016 for PPR. k, Pooled data of evoked EPSCs and PPR in the shRNA-Pmch group, n= 12/3 neurons/animals. Paired two-tailed Student’s t-tests were used, P= 0.774 for eEPSCs and P= 0.4796 for PPR. Data are mean ± s.e.m. *P< 0.001. Credit: Liu et al. (自然神经科学, 2022)。
